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SRX10341594: GSM5172109: RM532_3; Zymomonas mobilis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 9.6M spots, 2.9G bases, 929Mb downloads

Submitted by: NCBI (GEO)
Study: Investigated the responses of ZM532 and wild-type ZM4 to acetic acid and furfural by using RNA-seq
show Abstracthide Abstract
In our previous study, we successfully constructed an engineered Zymomonas mobilis ZM532 strain tolerant these double inhibitors by genome shuffling, but the molecular mechanisms of tolerance to these inhibitors are still unknown. The goal of this study investigated the responses of ZM532 and wild-type ZM4 to acetic acid and furfural using Transcriptome Overall design: ZM532 and wild-type ZM4 cultured in RM and AF medium in triplicate and then extracted the RNA. The concentrations of acetic acid and furfural combinations were set at 5.0 g/L and 3.0 g/L to study the responses of ZM4 and mutant ZM532.
Sample: RM532_3
SAMN18311068 • SRS8460803 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNAs were extracted using the RNA isolation kit from bacteria cells (2 strains × 2 conditions × 3 replications) cultured in RM and AF medium (Tiangen, China). Highly qualified RNAs were used for construction and sequencing of cDNA libraries. The high-throughput sequencing was conducted by Illumina Hiseq 2000 platform after passing via a number of screening phases
Experiment attributes:
GEO Accession: GSM5172109
Links:
Runs: 1 run, 9.6M spots, 2.9G bases, 929Mb
Run# of Spots# of BasesSizePublished
SRR139636039,604,5902.9G929Mb2021-03-18

ID:
13523857

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