GSM1686480: P21_Mfn2AA_10534-4; Mus musculus; RNA-Seq - SRA

SRX1029345: GSM1686480: P21_Mfn2AA_10534-4; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 21.9M spots, 1.1G bases, 772.5Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Parkin-mediated mitophagy evokes perinatal cardiac mitochondria maturation

PRJNA284112 • SRP058378 • All experiments • All runs

show Abstracthide Abstract

Exclusion of Parkin from mitochondria of perinatal cardiomyocytes interrupts structural and molecular transformations essential to normal perinatal-adult mitochondrial replacement. mRNA-sequencing from cardiac total RNA was performed at P1, P21 and 5-week stages of nontransgenic (ntg) and human-Mfn2-overexpressing (Mfn2wt) hearts, and also of tet-off control (tetoff) and human-Mfn2 T111A/S442A-overexpressing (Mfn2AA) hearts. Overall design: Libraries from all P1 samples were prepared and analyzed together, and similarly all P21 libraries, and all 5-week libraries together. To facilitate comparison across time points by accounting for batch effect, new libraries were prepared starting from total RNA from selected P21 ntg and 5-week ntg hearts subjected to prior analysis, during the same batch preparation as all P1 samples, and analyzed together. Correction for differences observed between libraries prepared from the same total RNA, but at different times, allows comparison across timepoints.

Sample: P21_Mfn2AA_10534-4

SAMN03658662 • SRS937840 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Hearts were removed and ventricular apexes were flash frozen on dry ice, and RNA was harvested using Trizol reagent. Polyadenylated RNA was isolated using oligo(dT) binding, followed by chemical fragmentation and preparation of non-strand-specific, single-end RNA-Seq libraries, as per Methods Mol Biol. 2015;1299:27-49, PMID 25836573. non-strand-specific, single-end RNA-Seq

Experiment attributes:

GEO Accession: GSM1686480

Links:

External link: GEO Sample GSM1686480

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 21.9M spots, 1.1G bases, 772.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR2027935	21,927,742	1.1G	772.5Mb	2015-12-04

ID:: 1492557

SRA

Sequence Read Archive

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