GSM5135122: MEP scRNA; Mus musculus; RNA-Seq - SRA

SRX10240666: GSM5135122: MEP scRNA; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 265.6M spots, 26G bases, 12.8Gb downloads

Submitted by: NCBI (GEO)

Study: Identification and isolation of hematopoietic stem and progenitor cell populations (scRNA-seq)

PRJNA706739 • SRP309359 • All experiments • All runs

show Abstracthide Abstract

Single cell RNA sequencing of 37,501 cells representing four independently isolated hematopoietic stem and progenitor populations from mouse bone marrow Overall design: Single cell transcriptional profiling using the 10X Genomics platform was performed on 10,882 Lineage-depleated SCA1+ KIT+ cells, 12,185 common myeloid progenitors, 8,064 granulocyte/monocyte progenitors, and 6,370 megakaryocyte/erythroid progenitors independently isolated from normal mouse bone marrow

Sample: MEP scRNA

SAMN18142667 • SRS8382064 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was isolated and cDNA constructed using the 10x Genomics Single cell 3' Library and Gel Bead Kit according to the manufacturer's protocol. Libraries were constructed using Chromium Single Cell 3' library and Gel Bead Kits (10x Genomics) and sequencing performed on a HiSeq 2500 DNA Sequencer (with a 26 base read for cell and molecule barcodes, an 8 base library index read, and a 98 base insert read)

Experiment attributes:

GEO Accession: GSM5135122

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 265.6M spots, 26G bases, 12.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR13859684	265,559,103	26G	12.8Gb	2023-04-03

ID:: 13368242

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