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SRX10240664: GSM5135120: CMP scRNA; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 444.2M spots, 43.5G bases, 21.1Gb downloads

Submitted by: NCBI (GEO)
Study: Identification and isolation of hematopoietic stem and progenitor cell populations (scRNA-seq)
show Abstracthide Abstract
Single cell RNA sequencing of 37,501 cells representing four independently isolated hematopoietic stem and progenitor populations from mouse bone marrow Overall design: Single cell transcriptional profiling using the 10X Genomics platform was performed on 10,882 Lineage-depleated SCA1+ KIT+ cells, 12,185 common myeloid progenitors, 8,064 granulocyte/monocyte progenitors, and 6,370 megakaryocyte/erythroid progenitors independently isolated from normal mouse bone marrow
Sample: CMP scRNA
SAMN18142669 • SRS8382063 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was isolated and cDNA constructed using the 10x Genomics Single cell 3' Library and Gel Bead Kit according to the manufacturer's protocol. Libraries were constructed using Chromium Single Cell 3' library and Gel Bead Kits (10x Genomics) and sequencing performed on a HiSeq 2500 DNA Sequencer (with a 26 base read for cell and molecule barcodes, an 8 base library index read, and a 98 base insert read)
Experiment attributes:
GEO Accession: GSM5135120
Links:
Runs: 1 run, 444.2M spots, 43.5G bases, 21.1Gb
Run# of Spots# of BasesSizePublished
SRR13859682444,248,24043.5G21.1Gb2023-04-03

ID:
13368240

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