U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX1014853: GSM1671927: Subject4_Cervical; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 396,565 spots, 18.2M bases, 12.1Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Role of cervicovaginal microbiota in genital inflammation
show Abstracthide Abstract
We performed gene expression analysis using the SCRB-Seq method from cervical and peripheral blood antigen presenting cells that were collected from women with different cervicovaginal bacterial community types. Overall design: Cervical and peripheral blood antigen presenting cells were FACS sorted using the gating strategy: FSC vs. SSC -> Singlets -> Live CD45+ -> HLA-DR+ -> CD11c+ or CD14+. The genital bacterial microbiomes were characterized in the same women and categorized into Lactobacillus dominance, Gardnerella dominance, or Prevotella dominance.
Sample: Subject4_Cervical
SAMN03577736 • SRS926513 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Cells were sorted directly into TriZOL on a FACS Aria III and frozen at -80C. Gen-Elute linear polyacrylamide (Sigma) was added to the RNA in TRIzol, followed by the addition of 0.2 volumes of chloroform and vortexing. After centrifuging at 14,000xg for 5 minutes, the aqueous phase was transferred to a clean tube. Nucleic acid was precipitated with isopropanol and 3M sodium acetate pH 5.5 (Ambion) at -20°C overnight and then centrifuged at maximum speed for 30 minutes at 4°C. The supernatant was discarded and the pellet was washed by adding 0.5mL of 100% ethanol, centrifuging for 15 minutes at 4°C, and discarding the supernatant. The nucleic acid pellet was allowed to dry and was resuspended in 5 μL of molecular grade water. RNA-DGE derived from the Single Cell RNA Barcoding and Sequencing (SCRB-Seq) strategy 3’ Digital Gene Expression (3’ DGE)
Experiment attributes:
GEO Accession: GSM1671927
Links:
External link:
Runs: 1 run, 396,565 spots, 18.2M bases, 12.1Mb
Run# of Spots# of BasesSizePublished
SRR2003803396,56518.2M12.1Mb2015-05-20

ID:
1470974

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...