Instrument: NextSeq 500
Strategy: ATAC-seq
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: Kidney paracancerous tissues was stored in PBS on ice and then cut into 1mm3 segments for nuclei isolation. Samples were homogenized using a Dounce homogenizer in 1ml chilled Lysis Buffer on ice. Following lysis, 9 ml chilled Wash Buffer was added and the resulting solution was filtered through a 30-µm cell strainer. Nuclei were centrifuged (500g for 5 min at 4 °C) and the supernatant removed without disrupting the nuclei pellet. Nuclei were resuspended in chilled Diluted Nuclei Buffer at approximately 3,000–4,000 nuclei per µl. 10X Chromium libraries were prepared according to manufacturer protocol.