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SRX013983: DBY11331 whole genome sequencing CA0001.SAM2.EXP2
1 ILLUMINA (Illumina Genome Analyzer) run: 7.5M spots, 269.5M bases, 186Mb downloads

Design: Briefly, 10 ug of yeast genomic DNA were sonicated to fragment sizes below 2000 bp, concentrated and end-r epaired using the End-It DNA repair kit (EPICENTRE Biotechnologies). End-repaired DNA was A-tailed with GoTaq DNA polymerase (Promega) and ligated t o Illumina adapters (QuickLigase, NEB). Ligation products between 300-400bp were excised from a 6% polyacrylamide gel, eluted and ethanol precipitat ed. Fragment libraries were PCR amplified, cleaned following AMPure (Agencourt) and Qiaquick PCR clean-up procedures, and submitted for sequencing. We prepared two such libraries for each strain. We collected 13,555,852 and 13,901,121 single-end, 36 bp, quality-filtered reads from DBY11331 and DBY10147, respectively, using the Illumina Genome Analyzer II platform.
Submitted by: University of Washington Genome Sciences (UWGS-FL)
Study: Whole-genome sequencing of a laboratory-evolved yeast strain CA0001
show Abstracthide Abstract
We sequenced >93.5% of the genome of a laboratory-evolved strain of the yeast Saccharomyces cerevisiae and its ancestor at >28x depth. Both single nucleotide polymorphisms and copy number amplifications were found, with specific gains over array-based methodologies previously used to analyze these genomes. Applying a segmentation algorithm to quantify structural changes, we determined the approximate genomic boundaries of a 5x gene amplification. These boundaries guided the recovery of breakpoint sequences, which provide insights into the nature of a complex genomic rearrangement.
Sample: Generic sample from Saccharomyces cerevisiae
SAMN00006200 • SRS008328 • All experiments • All runs
Library:
Name: DBY11331_CA0001.SAM2.EXP2
Instrument: Illumina Genome Analyzer
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Spot descriptor:
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Runs: 1 run, 7.5M spots, 269.5M bases, 186Mb
Run# of Spots# of BasesSizePublished
SRR0312597,485,708269.5M186Mb2009-12-10

ID:
14304

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