Illumina sequencing of Mouse MCV6_H3K4me3_ChIPSeq genomic fragment li... - SRA

SRX000413: Illumina sequencing of Mouse MCV6_H3K4me3_ChIPSeq genomic fragment library
2 ILLUMINA (Illumina Genome Analyzer) runs: 18.9M spots, 682.1M bases, 4.7Gb downloads

Design: Described in Mikkelsen et al., Nature 2007. Briefly, chromatin was fixed in 1% formaldehyde, sheared to 200-700bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.

Submitted by: Gene Expression Omnibus (GEO)

Study: Chromatin state maps (H3K4me3 and H3K27me3) from partially and fully reprogrammed mouse cell lines

PRJNA107063 • SRP000215 • All experiments • All runs

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Chromatin state maps (H3K4me3 and H3K27me3) from partially and fully reprogrammed mouse cell lines obtained by ectopic expression of Oct4, Sox2, Klf4 and c-Myc using constitutive retroviral infection of MEFs (MCV6, MCV8, MCV8.1) or induction of lentivirus in secondary B lymphocytes obtained from iPS-derived chimeric mice (BIV8). Keywords: High-throughput ChIP-sequencing, Illumina, cell type comparison Overall design: H3K4me3 and H3K27me3 ChIP-Seq in singlicate from three partially reprogrammed cell lines (BIV1, MCV8, MCV6), one iPS cell line (MCV8.1) and MEFs (subsampled from Mikkelsen et al, Nature, 2007))

Sample: Generic sample from Mus musculus

SAMN00000288 • SRS000506 • All experiments • All runs

Organism: Mus musculus

Library:

Name: MCV6_H3K4me3_ChIPSeq

Instrument: Illumina Genome Analyzer

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 2 runs, 18.9M spots, 682.1M bases, 4.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR001642	12,385,191	445.9M	3Gb	2008-06-26
SRR029192	6,561,051	236.2M	1.6Gb	2009-10-28

ID:: 414

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