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SRX043505: Illumina Transcriptome Sequencing of D. melanogster chemical treatment: Cd Treatment 0.05mM CdCl2 Late Third Instar Larvae 6hr
4 ILLUMINA (Illumina Genome Analyzer II) runs: 87.2M spots, 13.3G bases, 4.9Gb downloads

Design: Libraries were paired-end sequenced on an Illumina Genome Analyzer II
Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Drosophila melanogaster strain:y; cn bw sp Transcriptome or Gene expression
show Abstracthide Abstract
RNA-seq analysis was performed on poly(A) RNA from D. melanogaster treated with various chemicals through feeding, subjected to temperature shock, or exposure to viruses. Total RNA was isolated by the Peter Cherbas group. Isolation of poly(A) RNA and strand-specific library construction were performed in the Brenton Graveley lab. Libraries were distributed among 3 labs in the Drosophila Transcriptome group for sequencing (Celniker, Gingeras, and Graveley) for paired-end RNA sequencing (2x76 nt) on the GAIIx and HiSeq platforms. Fastq files were generated using pipeline version 1.5. Reads were aligned to the genome and a splice-junction database using Bowtie 0.12.0-beta1 and (to identify only uniquely-mapped reads).
Sample: D. melanogster chemical treatment: Cd Treatment 0.05mM CdCl2 Late Third Instar Larvae 6hr
SAMN00215704 • SRS172635 • All experiments • All runs
Name: D.mel. Cd_0.05M_Cdcl2_L3_6hr
Instrument: Illumina Genome Analyzer II
Strategy: EST
Selection: cDNA
Layout: PAIRED
Spot descriptor:
forward77  reverse

Runs: 4 runs, 87.2M spots, 13.3G bases, 4.9Gb
Run# of Spots# of BasesSizePublished


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