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SRX008235: Illumina Transcriptome Sequencing of D. melanogaster embryos, 12-14 hours after egg laying
2 ILLUMINA (Illumina Genome Analyzer II) runs: 32.3M spots, 2.5G bases, 1.6Gb downloads

Design: Libraries were single-end sequenced on an Illumina Genome Analyzer II in the laboratory of T. Gingeras
Submitted by: Berkeley Drosophila Genome Project (BDGP)
Study: Developmental Time Course poly(A)+ RNA Profiling in D. melanogaster
show Abstracthide Abstract
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.
Sample: Generic sample from Drosophila melanogaster
SAMN00003029 • SRS004674 • All experiments • All runs
Library:
Name: D.mel. embryos, 12-14 hr
Instrument: Illumina Genome Analyzer II
Strategy: EST
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Spot descriptor:
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Runs: 2 runs, 32.3M spots, 2.5G bases, 1.6Gb
Run# of Spots# of BasesSizePublished
SRR03522116,717,9021.3G858Mb2010-02-03
SRR02370315,588,3281.2G787Mb2009-09-15

ID:
8521

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