show Abstracthide AbstractThe study of insect symbiotic bacteria can provide us with a better understanding of their roles in insect physiology, behavior and genetics. Although some progress has been made in the study of insect gut microbes, there is still a lack of understanding of microbial diversity in other areas. In this study, we describe a bacterial 16S rRNA amplification dataset extracted from salivary glands, gut tissues, sperms and ovaries of hytophagous Halyomorpha halys and predatory Arma chinensis. The raw data was obtained by sequencing V3-V4 region of 16S rRNA gene and comprised 4,574,879 paired-end raw reads generated from 40 samples. The results of this study provide data on microbial diversity of two species of bugs with different feeding characteristics in a laboratory population with a single food source, laying a foundation for further understanding of the microbial symbiosis between A. chinensis and H. halys, and may provide new ideas for the development of biological control strategies.