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SRX892008: Sexually Active Male Gonad of Brevoortia tyrannus
1 ILLUMINA (Illumina HiSeq 2000) run: 36.3M spots, 9G bases, 3.7Gb downloads

Design: The dUTP-strand specific cDNA library was made using chemical hydrolysis and the Illumina Ultra Directional RNA-seq kit (NEB). Libraries were barcoded with Tru-Seq adaptors and amplified with 12-15 cycles of PCR (Illumina). Completed RNAseq libraries were quantified with Qubit DNA HS, BioAnalyzer High Sensitivity DNA (Invitrogen, Agilent, KAPA). The libraries were sequenced using the NextSeq 500 High Output 300 cycles kit, reading 155 x 155 bp Paired End Sequencing.
Submitted by: Seton Hall University
Study: Atlantic Menhaden (Brevoortia tyrannus) raw sequence reads of testes
show Abstracthide Abstract
Development of molecular biomarkers for monitoring menhaden
Sample: Sexually Active Male Gonad of Brevoortia tyrannus
SAMN03378155 • SRS858218 • All experiments • All runs
Name: Menhaden #1 Gonad
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: PolyA
Layout: PAIRED
Spot descriptor:
forward156  reverse

Runs: 1 run, 36.3M spots, 9G bases, 3.7Gb
Run# of Spots# of BasesSizePublished


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