GSM1616861: Cell22; Mus musculus; RNA-Seq - SRA

SRX886051: GSM1616861: Cell22; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 2.1M spots, 197M bases, 103.3Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Intrinsic age-dependent changes and cell-cell contacts regulate nephron progenitor lifespan

PRJNA276103 • SRP055425 • All experiments • All runs

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Purpose: To use single-cell RNA-Seq analysis of nephron progenitors in order to determine transcrptional differences as nephron progenitors age. Methods: Using a combination of FACS sorting and a Fluidigm Single-cell auto-prep system, we generated high-throughput RNA-SEQ data of nephron progenitors during development Results: Single cells transcriptome profiling of nephron progenitors revealed progressive age-dependent changes with heterogeneity increasing in older populations. Overall design: 96-single cell transcriptomes were determined from nephron progenitors of e14.5, e18.5 and P0 using Cited1GFP transgenic animals

Sample: Cell22

SAMN03366436 • SRS852902 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Single-cell were loaded onto a Fluidigm Single-cell 10uM-17uM chip using the C-1 Autoprep system. Cells were lysed and cDNAs generated following manufacturer's protocol. The construction of RNA libraries were generated using Illumina Tagmentation following manufactuer's protocols

Experiment attributes:

GEO Accession: GSM1616861

Links:

External link: GEO Sample GSM1616861

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 2.1M spots, 197M bases, 103.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR1813644	2,087,611	197M	103.3Mb	2015-12-23

ID:: 1272910

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