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SRX663331: GSM1447120: WT7; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 43.6M spots, 2.2G bases, 1.5Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Transcriptome analysis of isolated stormal cells and tumor epithelial cells in mouse lung cancer by RNA-Seq
show Abstracthide Abstract
We sequenced mRNA from individual stormal cells (Macrophages, Monocytes, and Neutrophils) and tumor epithelial cells from KrasG12dD; p53-/- murine lung cancer model and WT control mouse to compare gene expressio profiles of lung cancer stroma and tumor cells to their counterparts of WT lugns. The tumor was generated by injecting HKP1 lung cancer cell line, which was driven by KrasG12D activation and loss of p53, via tail vein. The cells were sorted by their specific surface markers at day 20-25 after orthortopic lung cancer formation. Overall design: Examination of mRNA levels in individual stormal cells and tumor cells from tumor lungs compared to their counterparts from WT lungs
Sample: WT7
SAMN02943134 • SRS668204 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: Lungs were purfused, removed, and dissociated to make single suspension. After staining with proper antibody of surface markers, cells were sorted usign BD Aria II sorter. The RNA was harvested from the sorted cells using Arcturus PicoPure RNA isolation kit. Illumina TruSeq RNA Sample Prep Kit v2 (Cat#RS-122-2001) was used with 16-33ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols(Illumina TruSeq RNA Sample Prep Kit v2 (Cat#RS-122-2001)).
Experiment attributes:
GEO Accession: GSM1447120
External link:
Runs: 1 run, 43.6M spots, 2.2G bases, 1.5Gb
Run# of Spots# of BasesSizePublished


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