Resistance gene enrichment sequencing (RenSeq) of C. annuum 20 - SRA

SRX4891011: Resistance gene enrichment sequencing (RenSeq) of C. annuum 20
1 PACBIO_SMRT (Sequel) run: 165,058 spots, 439.5M bases, 345.3Mb downloads

Design: Genomic DNA of young leaf tissues were extracted using DNeasy Plant Mini Kit (Qiagen, Germany). 10 ug of gDNA were fragmented with the Covaris sonicator (Covaris Inc., MA, USA) and size-selected with Sage-ELF (Sage Science, MA, USA) to get 2-5 kb fragments. The libraries were generated with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, MA, USA). To capture the NLR gene fragments, customized MYbaits kit (Arbor Biosciences, MI, USA) was used. The captured libraries were amplified using PCR (KAPA HiFi enzyme) and size-selected with Sage-ELF again. The enriched library was sequenced using PacBio Sequle in a SMART cell at The Vincent J. Coates Genomics Sequencing Laboratory (GSL) at the University of California, Berkeley. The circular consensus sequence (CCS) reads were generated using the SMRT portal software (minimum 3 full passes and 90% accuracy). The CCS reads were de-multiplexed to to produce the CCS reads of C. annuum 20 (index primers: P707 and P503)

Submitted by: University of California Berkeley

Study: Resistance gene enrichment sequencing (RenSeq) of Solanaceae

PRJNA496490 • SRP165809 • All experiments • All runs

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The disease resistance genes encoding NBS-LRR proteins in the Solanaceae family, mainly tomatoes, were selectively captured, enriched and sequenced using resistance gene enrichment sequencing (RenSeq) and PacBio

Sample: C. annuum 20

SAMN10243992 • SRS3938292 • All experiments • All runs

Organism: Capsicum annuum

Library:

Name: C. annuum 20

Instrument: Sequel

Strategy: Targeted-Capture

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Runs: 1 run, 165,058 spots, 439.5M bases, 345.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR8061497	165,058	439.5M	345.3Mb	2018-10-16

ID:: 6591450

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