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SRX4891010: Resistance gene enrichment sequencing (RenSeq) of N. benthamiana
1 PACBIO_SMRT (Sequel) run: 36,738 spots, 121.4M bases, 98.4Mb downloads

Design: Genomic DNA of young leaf tissues were extracted using DNeasy Plant Mini Kit (Qiagen, Germany). 10 ug of gDNA were fragmented with the Covaris sonicator (Covaris Inc., MA, USA) and size-selected with Sage-ELF (Sage Science, MA, USA) to get 2-5 kb fragments. The libraries were generated with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, MA, USA). To capture the NLR gene fragments, customized MYbaits kit (Arbor Biosciences, MI, USA) was used. The captured libraries were amplified using PCR (KAPA HiFi enzyme) and size-selected with Sage-ELF again. The enriched library was sequenced using PacBio Sequle in a SMART cell at The Vincent J. Coates Genomics Sequencing Laboratory (GSL) at the University of California, Berkeley. The circular consensus sequence (CCS) reads were generated using the SMRT portal software (minimum 3 full passes and 90% accuracy). The CCS reads were de-multiplexed to to produce the CCS reads of N. benthamiana (P712 and P508)
Submitted by: University of California Berkeley
Study: Resistance gene enrichment sequencing (RenSeq) of Solanaceae
show Abstracthide Abstract
The disease resistance genes encoding NBS-LRR proteins in the Solanaceae family, mainly tomatoes, were selectively captured, enriched and sequenced using resistance gene enrichment sequencing (RenSeq) and PacBio
Sample: N. benthamiana
SAMN10243991 • SRS3938291 • All experiments • All runs
Library:
Name: N. benthamiana
Instrument: Sequel
Strategy: Targeted-Capture
Source: GENOMIC
Selection: PCR
Layout: SINGLE
Runs: 1 run, 36,738 spots, 121.4M bases, 98.4Mb
Run# of Spots# of BasesSizePublished
SRR806149836,738121.4M98.4Mb2018-10-16

ID:
6591449

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