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SRX4717057: RNA-Seq of human Vdelta1 T cells from peripheral blood. Donor 6, delta chain
1 ILLUMINA (Illumina MiSeq) run: 158,279 spots, 66M bases, 39Mb downloads

Design: Flow cytometric sorted cells were subjected to RNA extraction using the RNAeasy mini kit (Qiagen). Next, 12l mRNA of each sample was reverse transcribed with the Superscript III enzyme kit (Invitrogen). CDR3 TRG and TRD sequencing amplicons were generated as described previously (Ravens et al. Nat Immunology 2017), while using 25 30 PCR cycles. According to Illumina guidelines 96-samples were labeled with Nextera XT indices and subjected to Illumina MiSeq analysis (500 cycle; paired-end sequencing), while 20% PhIX was added as a control.
Submitted by: Instituto de Medicina Molecular Joao Lobo Antunes
Study: HTS data of human gamma and delta repertoires from peripheral blood, DOT and naive vs differentiated V delta 1 T cells
show Abstracthide Abstract
This study aims at characterizing the gamma and delta repertoires of human V delta 1 T cells of different origins:- Sorted Vdelta1 T cells from peripheral blood;- Sorted Vdelta1 T cells from DOT cells (Almeida, Clin Cancer Res, 2016);- Sorted naive CD27+ naive and CD27- differentiated Vdelta1 Tcells from peripheral blood.
Sample:
SAMN10092809 • SRS3802698 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: HD6_PB_delta
Instrument: Illumina MiSeq
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 158,279 spots, 66M bases, 39Mb
Run# of Spots# of BasesSizePublished
SRR7878388158,27966M39Mb2019-03-14

ID:
6384887

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