RNA-Seq of Elaeis guineensis: mature tree leaf - SRA

SRX4663593: RNA-Seq of Elaeis guineensis: mature tree leaf
1 ILLUMINA (Illumina HiSeq 4000) run: 23.7M spots, 7.1G bases, 2.7Gb downloads

Design: After the QC procedures using Nanodrop, Agarose Gel Electrophoresis and Agilent 2100, mRNA from eukaryotic organisms is enriched using oligo(dT) beads. For prokaryotic samples, rRNA is removed using a specialized kit that leaves the mRNA. The mRNA from either eukaryotic or prokaryotic sources is then fragmented randomly in fragmentation buffer, followed by cDNA synthesis using random hexamers and reverse transcriptase. After first-strand synthesis, a custom second-strand synthesis buffer (Illumina) is added with dNTPs, RNase H and Escherichia coli polymerase I to generate the second strand by nick-translation. The final cDNA library is ready after a round of purification, terminal repair, A-tailing, ligation of sequencing adapters, size selection and PCR enrichment.

Submitted by: Indonesian Research Institute for Biotechnology and Bioindustry

Study: Development of clonal oil palm material tolerant to Ganoderma using genome editing

PRJNA490055 • SRP160527 • All experiments • All runs

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This research aims to develop palm clonal plant tolerant to Ganoderma. This research uses the genome editing approach through the CRISPR-Cas9 system. In the early stages, RNAseq analysis was conducted on tolerant and susceptible plants against Ganoderma to identify key gene expression related to tolerance traits.

Sample:

SAMN10023964 • SRS3758778 • All experiments • All runs

Organism: Elaeis guineensis

Library:

Name: Elaeis-IRIBB-Socfindo-1

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 23.7M spots, 7.1G bases, 2.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR7812015	23,733,515	7.1G	2.7Gb	2018-09-10

ID:: 6321300

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