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SRX3230256: small RNA seq from control and cold treated Arabidopsis roots
1 ILLUMINA (Illumina HiSeq 4000) run: 11.2M spots, 275.4M bases, 128.2Mb downloads

Design: Small RNA with lengths of 18Ð30 nt were separated and purified using denaturing polyacrylamide gel electrophoresis. Followed by sequential 3Õ and 5Õ RNA adaptor ligation to the small RNAs using T4 RNA Ligase. The adaptor ligated samples were then reverse transcribed and amplified by PCR to construct the final libraries. Then, the prepared libraries were sequenced using an Illumina HiSeq 4000 platform
Submitted by: Iwate University
Study: Auxin regulated cold responsive small RNA
show Abstracthide Abstract
The current study is based on utilization of miRNA and unfold the role of miRNA in auxin mediated cold stress tolerance mechanism in plants.
Sample: small RNA seq from control and cold treated Arabidopsis roots
SAMN07718363 • SRS2554765 • All experiments • All runs
Library:
Name: slr1_4_
Instrument: Illumina HiSeq 4000
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: RT-PCR
Layout: SINGLE
Runs: 1 run, 11.2M spots, 275.4M bases, 128.2Mb
Run# of Spots# of BasesSizePublished
SRR611752711,236,209275.4M128.2Mb2017-10-01

ID:
4539336

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