small RNA seq from control and cold treated Arabidopsis roots - SRA

SRX3230254: small RNA seq from control and cold treated Arabidopsis roots
1 ILLUMINA (Illumina HiSeq 4000) run: 11.4M spots, 273M bases, 132.1Mb downloads

Design: Small RNA with lengths of 18Ð30 nt were separated and purified using denaturing polyacrylamide gel electrophoresis. Followed by sequential 3Õ and 5Õ RNA adaptor ligation to the small RNAs using T4 RNA Ligase. The adaptor ligated samples were then reverse transcribed and amplified by PCR to construct the final libraries. Then, the prepared libraries were sequenced using an Illumina HiSeq 4000 platform

Submitted by: Iwate University

Study: Auxin regulated cold responsive small RNA

PRJNA412581 • SRP119117 • All experiments • All runs

show Abstracthide Abstract

The current study is based on utilization of miRNA and unfold the role of miRNA in auxin mediated cold stress tolerance mechanism in plants.

Sample: small RNA seq from control and cold treated Arabidopsis roots

SAMN07718363 • SRS2554765 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Name: Col0_4_

Instrument: Illumina HiSeq 4000

Strategy: miRNA-Seq