GSM1183061: XT32_ctrl_polyA-RNA_3rd; Xenopus tropicalis; RNA-Seq - SRA

SRX319538: GSM1183061: XT32_ctrl_polyA-RNA_3rd; Xenopus tropicalis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 86.9M spots, 9.6G bases, 4.6Gb downloads

Submitted by: NCBI (GEO)

Study: In vivo T-box Transcription Factor Profiling Reveals Joint Regulation of Embryonic Neuro-mesodermal Bipotency

PRJNA210967 • SRP026685 • All experiments • All runs

show Abstracthide Abstract

Brachyury (Xbra/Xbra3) knock-down embryos of the frog Xenopus tropicalis were profiled to quantify neuro-mesodermal cell fate switches at a transcriptional level. Overall design: Transcriptional profiling of Xbra/Xbra3 double morphants at early tadpole stage (RNA-Seq) in biological triplicates.

Sample: XT32_ctrl_polyA-RNA_3rd

SAMN02230360 • SRS456186 • All experiments • All runs

Organism: Xenopus tropicalis

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Embryos were flash-frozen on dry ice, and total RNA was extracted using Trizol (Invitrogen) and subjected to two precipitations with ethanol and LiCl. Total RNA to the amount of two X. tropicalis embryos (~3 µg) was processed according to the Illumina TruSeq protocol (November 2010). The quality was controlled on the Bioanalyser 2100 (Agilent) using the DNA-specific chip DNA 1000 (Agilent). Libraries were read paired-end along 55 bases on the Illumina HiSeq 2000 machine.

Experiment attributes:

GEO Accession: GSM1183061

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 86.9M spots, 9.6G bases, 4.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR929124	86,917,829	9.6G	4.6Gb	2015-07-22

ID:: 446489

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