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SRX318125: Megagametophyte RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 77.2M spots, 15.4G bases, 9.3Gb downloads

Design: Total RNA was extracted from pool of 10 megagametophytes of White spruce (PG29) using Dynabeads mRNA direct micro kit from Invitrogen. Quality and quantity was checked using Agilent 2100 BioAnalyzer. The libraries were constructed at the British Columbia Genome Sciences Centre (GSC). The 350-750bp (average <500bp) fractions were made into libraries using RNAseq chemistry; GSC protocol SLX-IPET. Fragments were then indexed (TAGCTT), paired end, and sequenced by Illumina HiSeq-3 with a read length of 100bp. The goal of the experiment is to study the gene richness and composition specific to megagametophyte tissue from white spruce.
Submitted by: Michael Smith Labs, University of British Columbia
Study: Picea glauca RNA-Seq
show Abstracthide Abstract
Whole transcriptome sequencing of various tissues of White Spruce PG29.
Sample: Generic sample from Picea gluaca
SAMN02223065 • SRS455075 • All experiments • All runs
Organism: Picea glauca
Library:
Name: Megagametophyte
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Spot descriptor:
forward101  reverse

Runs: 1 run, 77.2M spots, 15.4G bases, 9.3Gb
Run# of Spots# of BasesSizePublished
SRR92625177,214,46315.4G9.3Gb2013-07-16

ID:
443668

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