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SRX318120: Mature Needle RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 110.8M spots, 33.2G bases, 19.9Gb downloads

Design: Total RNA was extracted from the mature needles of White spruce (PG29) using methodology from Kolosova et al 2004. Quality and quantity was checked using Agilent 2100 BioAnalyzer. The libraries were constructed at the British Columbia Genome Sciences Centre (GSC). The 300bp fractions were made into libraries using RNAseq chemistry; GSC protocol SLX-IPET. Fragments were then indexed (GCCAAT), paired end, and sequenced by Illumina HiSeq-10 with a read length of 150bp. The goal of the experiment is to study the gene richness and composition specific to mature needle tissue from white spruce.
Submitted by: Michael Smith Labs, University of British Columbia
Study: Picea glauca RNA-Seq
show Abstracthide Abstract
Whole transcriptome sequencing of various tissues of White Spruce PG29.
Sample: Generic sample from Picea gluaca
SAMN02223060 • SRS455070 • All experiments • All runs
Organism: Picea glauca
Library:
Name: Mature Needle
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Spot descriptor:
forward151  reverse

Runs: 1 run, 110.8M spots, 33.2G bases, 19.9Gb
Run# of Spots# of BasesSizePublished
SRR926246110,790,93233.2G19.9Gb2013-07-16

ID:
443663

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