Flush Bud RNA-Seq - SRA

SRX318119: Flush Bud RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 123.4M spots, 37G bases, 23.2Gb downloads

Design: Total RNA was extracted from the flushed buds of White spruce (PG29) using methodology from Kolosova et al 2004. Quality and quantity was checked using Agilent 2100 BioAnalyzer. The libraries were constructed at the British Columbia Genome Sciences Centre (GSC). The 300bp fractions were made into libraries using RNAseq chemistry; GSC protocol SLX-IPET. Fragments were then indexed (TTAGGC), paired end, and sequenced by Illumina HiSeq-10 with a read length of 150bp. The goal of the experiment is to study the gene richness and composition specific to flushing bud tissue from white spruce.

Submitted by: Michael Smith Labs, University of British Columbia

Study: Picea glauca RNA-Seq

PRJNA210511 • SRP026551 • All experiments • All runs

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Whole transcriptome sequencing of various tissues of White Spruce PG29.

Sample: Generic sample from Picea gluaca

SAMN02223059 • SRS455069 • All experiments • All runs

Organism: Picea glauca

Library:

Name: Flush Bud

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward151 reverse

Runs: 1 run, 123.4M spots, 37G bases, 23.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR926245	123,419,853	37G	23.2Gb	2013-07-16

ID:: 443662

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