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SRX3176762: small_RNA-Seq_15_days_seedlings_flg22
1 ILLUMINA (Illumina HiSeq 2500) run: 82.2M spots, 4.1G bases, 1.3Gb downloads

Design: Arabidopsis Columbia (Col-0) seedlings were grown on plates containing Murashige and Skoog (MS) medium (composition for a 1 L medium (pH = 5.7) : 2.3 g MS, 0.5% sucrose, 0.8% agar, 0.5 g MES, vitamins, 0.5% sucrose and 0.8 % agar, 0.5 g MES) under 16 h light condition at 22.5 °C and 8 h dark condition at 19 °C (Percival growth chamber AR-36L2). Seedlings were then transferred into 1/2 MS liquid medium at 12 days post-germination (2 seedlings per/500 _L of medium in wells of 24-well plates). Forty eight hours after seedling transfer, the medium was supplied with the flg22 peptide QRLSTGSRINSAKDDAAGLQIA (final concentration of 10 _M). Seedling samples were further collected at 60 min after treatment and frozen into liquid nitrogen. RNA extraction was done with Trizol.
Submitted by: IBENS
Study: Arabidopsis thaliana Transcriptome or Gene expression
show Abstracthide Abstract
Analysis of Arabidopsis microRNA expression and regulation in response to flg22.
Sample: small_RNA-Seq_15_days_seedlings_flg22
SAMN07628946 • SRS2506455 • All experiments • All runs
Name: CF-2
Instrument: Illumina HiSeq 2500
Strategy: miRNA-Seq
Selection: PCR
Layout: SINGLE
Runs: 1 run, 82.2M spots, 4.1G bases, 1.3Gb
Run# of Spots# of BasesSizePublished


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