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SRX3081224: small RNA sequencing from A. thaliana grafts to analyze mobility
1 ILLUMINA (Illumina HiSeq 2500) run: 6.6M spots, 202.9M bases, 138.2Mb downloads

Design: Total RNA was extracted from rootstocks and scions of grafted Arabidopsis plants. Small RNAs were enriched using a mirVana column, and libraries were prepared with the NEBNext small RNA kit. Samples were seqenced on an Illumina HiSeq 2500 at the University of Missouri DNA Core Facility. Adapters were trimmed by the sequencing facility.
Submitted by: University of Arizona
Study: Analysis of small RNA mobility in Arabidopsis grafts
show Abstracthide Abstract
Transgenic Arabidopsis lines expressing AGO4 mutants with altered nucleocytoplasmic localization were grafted to dcl2,3,4 mutants to analyze the impact of cytoplasmic AGO4 on small RNA mobility. It is demonstrated that reduced cytoplasmic small RNA accumulation negatively impacts small RNA mobility.
Sample: GFP-AGO4_3/dcl2,3,4_3
SAMN07485814 • SRS2419857 • All experiments • All runs
Library:
Name: JG36
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Runs: 1 run, 6.6M spots, 202.9M bases, 138.2Mb
Run# of Spots# of BasesSizePublished
SRR59203826,646,428202.9M138.2Mb2017-08-09

ID:
4365499

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