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SRX3081223: small RNA sequencing from A. thaliana grafts to analyze mobility
1 ILLUMINA (Illumina HiSeq 2500) run: 4.9M spots, 146.2M bases, 100.5Mb downloads

Design: Total RNA was extracted from rootstocks and scions of grafted Arabidopsis plants. Small RNAs were enriched using a mirVana column, and libraries were prepared with the NEBNext small RNA kit. Samples were seqenced on an Illumina HiSeq 2500 at the University of Missouri DNA Core Facility. Adapters were trimmed by the sequencing facility.
Submitted by: University of Arizona
Study: Analysis of small RNA mobility in Arabidopsis grafts
PRJNA397727 • SRP115113 • All experiments • All runs
show Abstracthide Abstract
Transgenic Arabidopsis lines expressing AGO4 mutants with altered nucleocytoplasmic localization were grafted to dcl2,3,4 mutants to analyze the impact of cytoplasmic AGO4 on small RNA mobility. It is demonstrated that reduced cytoplasmic small RNA accumulation negatively impacts small RNA mobility.
Sample: GFP-AGO4_2/dcl2,3,4_2
SAMN07485813 • SRS2419855 • All experiments • All runs
Organism: Arabidopsis thaliana
Library:
Name: JG35
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Runs: 1 run, 4.9M spots, 146.2M bases, 100.5Mb
Run# of Spots# of BasesSizePublished
SRR59203834,887,743146.2M100.5Mb2017-08-09

ID:
4365498

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