small RNA sequencing from A. thaliana grafts to analyze mobility - SRA

SRX3081223: small RNA sequencing from A. thaliana grafts to analyze mobility
1 ILLUMINA (Illumina HiSeq 2500) run: 4.9M spots, 146.2M bases, 100.5Mb downloads

Design: Total RNA was extracted from rootstocks and scions of grafted Arabidopsis plants. Small RNAs were enriched using a mirVana column, and libraries were prepared with the NEBNext small RNA kit. Samples were seqenced on an Illumina HiSeq 2500 at the University of Missouri DNA Core Facility. Adapters were trimmed by the sequencing facility.

Submitted by: University of Arizona

Study: Analysis of small RNA mobility in Arabidopsis grafts

PRJNA397727 • SRP115113 • All experiments • All runs

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Transgenic Arabidopsis lines expressing AGO4 mutants with altered nucleocytoplasmic localization were grafted to dcl2,3,4 mutants to analyze the impact of cytoplasmic AGO4 on small RNA mobility. It is demonstrated that reduced cytoplasmic small RNA accumulation negatively impacts small RNA mobility.

Sample: GFP-AGO4_2/dcl2,3,4_2

SAMN07485813 • SRS2419855 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Name: JG35

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Runs: 1 run, 4.9M spots, 146.2M bases, 100.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR5920383	4,887,743	146.2M	100.5Mb	2017-08-09

ID:: 4365498

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