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SRX2700685: S.mediterranea RNASeq
1 ILLUMINA (NextSeq 500) run: 508.9M spots, 46.3G bases, 16.5Gb downloads

Design: Animals of sexual S. mediterranea were homogenized in Trizol reagent (Invitrogen) on ice using a Miccra D-8 homogenizer (Miccra) and processed according to the Trizol reagent protocol. Following phase separation by chloroform addition, and isopropanol precipitation, the RNA pellet was dissolved in 350 ¾l RLT buffer and purified according to the RNeasy Mini Kit (Qiagen), including on-column DNase I digestion. The RNA was eluted in nuclease-free water (Ambion) and stored at -80 °C. The total RNA was quantified using a NanoDrop 1000 spectrophotometer and integrity was checked by capillary electrophoresis on a Bioanalyzer 2100 (Agilent) using the RNA 6000 Nano Kit and processed for stranded RNAseq library preparation and sequencing.
Submitted by: Max Planck Institute of Molecular Cell Biology and Genetics
Study: Schmidtea mediterranea strain:S2 Genome sequencing and assembly
Sample: Schmidtea mediterranea sexual strain S2F17
SAMN06660624 • SRS2094658 • All experiments • All runs
Name: smes_rnaseq
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 508.9M spots, 46.3G bases, 16.5Gb
Run# of Spots# of BasesSizePublished


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