MaRAP2-13OE_Line1 - SRA

SRX23341509: MaRAP2-13OE_Line1
1 ILLUMINA (Illumina NovaSeq 6000) run: 26.6M spots, 8G bases, 2.4Gb downloads

Design: Isolation of mRNA, fragmentation and priming was carried out using 500 ng of total RNA. Fragmented and primed mRNA was further subjected to first strand synthesis followed by second strand synthesis. The double stranded cDNA was purified using purification beads. Purified cDNA was end-repaired, adenylated and ligated to Illumina adapters followed by second strand excision using USER enzyme at 37 C for 15mins. Adapter ligated cDNA was purified using beads and was subjected to 11cycles for Indexing-(98C for 30 sec, cycling (98C for 10sec, 65C for 75sec) and 65C for 5min) and enrich the adapter-ligated fragments. Final PCR products (sequencing library) were purified with beads, followed by library quality control check. Illumina-compatible sequencing library were quantified by Qubit fluorometer and fragment size distribution was analyzed on TapeStation.

Submitted by: CSIR-Central Institute of Medicinal and Aromatic Plants

Study: Reference-based Transcriptome Analysis of Arabidopsis samples

PRJNA1066104 • SRP485059 • All experiments • All runs

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Reference-based comparative transcriptome analysis of Arabidopsis thaliana of MaMYB TF OE Line 1, MaMYB TF OE Line 2, MaRAP2 TF OE Line 1, MaRAP2 TF OE Line 2, WT Col-0 I, WT Col-0 II.

Sample: Mentha arvensis MaRAP2.13 overexpression line L1

SAMN39496466 • SRS20203657 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Name: SO_11011_RAP2_13_OE_Line1

Instrument: Illumina NovaSeq 6000

Strategy: OTHER

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Runs: 1 run, 26.6M spots, 8G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR27674165	26,632,423	8G	2.4Gb	2024-01-22

ID:: 31555566

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