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SRX155037: Transcriptome analysis of chilling stressed C. bungeana seedlings
4 ILLUMINA (Illumina HiSeq 2000) runs: 41.1M spots, 3.7G bases, 1.9Gb downloads

Design: Beads with Oligo(dT) are used to isolate poly(A) mRNA after total RNA is collected. Fragmentation buffer is added for interrupting mRNA to short fragments. Taking these short fragments as templates, random hexamer-primer is used to synthesize the first-strand cDNA. The second-strand cDNA is synthesized using buffer, dNTPs, RNaseH and DNA polymerase I, respectively. Short fragments are purified with QiaQuick PCR extraction kit and resolved with EB buffer for end reparation and adding poly(A). After that, the short fragments are connected with sequencing adapters. And, after the agarose gel electrophoresis, the suitable fragment are selected for the PCR amplification as templates.
Submitted by: LANZHOU UNIVERSITY
Study: Chorispora bungeana Transcriptome or Gene expression
show Abstracthide Abstract
Chorispora bungeana Transcriptome from 22C and 2C treated seedlings. mRNA libraries from 24 hours mock (22C) and cold (2C) treated seedlings of Chorispora bungeana were sequenced by Illumina sequencing. Transcriptome de novo assembly was carried out with SOAPdenovo software.
Sample: C. bungeana seedlings were treated at 2 degree (Celsius) for 24 hours
SAMN01055197 • SRS346265 • All experiments • All runs
Library:
Name: Chilling-stressed
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Spot descriptor:
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Runs: 4 runs, 41.1M spots, 3.7G bases, 1.9Gb
Run# of Spots# of BasesSizePublished
SRR51410310,266,667924M468.8Mb2012-11-23
SRR51479610,266,667924M456.3Mb2012-11-23
SRR51479710,266,667924M534.1Mb2012-11-23
SRR51479810,266,667924M525.1Mb2012-11-23

ID:
196221

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