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SRX154522: CH_EX_dsrB
1 LS454 (454 GS FLX Titanium) run: 8,259 spots, 3.5M bases, 8.6Mb downloads

Design: DNA samples were subject to 454 pyrotag sequencing as in (S E Dowd et al. 2008). Pyrotag sequencing employed dsrB primers DSR2060F 5’-CAACATCGTYCATACMCAGGG (Oakley et al. 2011) and DSR4R 5’-GTGTAGCAGTTACCGCA (M Wagner, A J Roger, et al. 1998). All samples were sequenced by a Roche 454 FLX instrument with titanium reagents following manufactures protocols (Roche Inc.). Initial generation of sequence library consisted of a one-step 25 cycle PCR with Hot Start plus Taq polymerase (Qiagen, Inc., Valencia, CA).
Submitted by: HARVARD UNIVERSITY
Study: Hydrothermal Deposit Targeted Locus (Loci)
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Understanding the distribution and activity of sulfate reduction at hydrothermal vents requires a more thorough interrogation of the community ecology and metabolic activity of vent hosted microorganisms in the context of the extreme physico-chemical environment. To date, many studies have quantified rates of sulfate reduction in hydrothermal-influenced sediments and vent isolated microorganisms, but only one study of sulfate reduction exists for hydrothermal deposits. Here we present rates of microbially-mediated sulfate reduction –as determined via 35S tracer studies- from three distinct hydrothermal deposits recovered from the Middle Valley vent field on the Juan de Fuca Ridge. We further evaluate these rates in the context of the in situ geochemistry and microbial community structure of each chimney to better constrain linkages between phylogeny and function. Middle Valley hydrothermal deposits Chowder Hill, Dead Dog, and Needles collected July 2010 AT15-67 on Alvin Dive 4625.
Sample: Chowder Hill_ Innoculum
SAMN01048206 • SRS345708 • All experiments • All runs
Library:
Name: Chowder Hill _ Ex_ dsrB
Instrument: 454 GS FLX Titanium
Strategy: AMPLICON
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Spot descriptor:
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Runs: 1 run, 8,259 spots, 3.5M bases, 8.6Mb
Run# of Spots# of BasesSizePublished
SRR5270578,2593.5M8.6Mb2013-06-13

ID:
195691

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