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SRX118021: Genome-wide DNA methylation data generated by MBD-isolated Genome Sequencing for prostate cell lines
2 ILLUMINA (Illumina Genome Analyzer IIx) runs: 30.9M spots, 1.1G bases, 768.2Mb downloads

Submitted by: CLEVELAND CLINIC FOUNDATION
Study: Dysregulation of cholesterol homeostasis in human prostate cancer through loss of ABCA1
show Abstracthide Abstract
Recent epidemiologic data show that low serum cholesterol level as well as statin use is associated with a decreased risk of developing aggressive or advanced prostate cancer, suggesting a role for cholesterol in aggressive prostate cancer development. Intracellular cholesterol promotes prostate cancer progression as a substrate for de novo androgen synthesis and through regulation of AKT signaling. By performing next-generation sequencing-based DNA methylome analysis, we have discovered marked hypermethylation at the promoter of the major cellular cholesterol efflux transporter, ABCA1, in LNCaP prostate cancer cells. ABCA1 promoter hypermethylation renders the promoter unresponsive to trans-activation and leads to elevated cholesterol levels in LNCaP. ABCA1 promoter hypermethylation is enriched in intermediate to high grade prostate cancers and not detectable in benign prostate. Remarkably, ABCA1 down-regulation is evident in all prostate cancers examined, and expression levels are inversely correlated with Gleason grade. Our results suggest cancer-specific ABCA1 hypermethylation and loss of protein expression direct high intracellular cholesterol levels and hence contribute to an environment conducive to tumor progression.
Sample: human prostate cancer cell line (LNCaP)
SAMN00779598 • SRS290482 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: LNCaP
Instrument: Illumina Genome Analyzer IIx
Strategy: MBD-Seq
Source: GENOMIC
Selection: MBD2 protein methyl-CpG binding domain
Layout: SINGLE
Spot descriptor:
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Runs: 2 runs, 30.9M spots, 1.1G bases, 768.2Mb
Run# of Spots# of BasesSizePublished
SRR40287914,499,854522M353.7Mb2012-12-19
SRR40288016,399,262590.4M414.5Mb2012-12-19

ID:
135948

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