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SRX1098347: 8369 Photinus pyralis male whole-genome shotgun sequences
1 ILLUMINA (Illumina HiSeq 2000) run: 66.5M spots, 13.4G bases, 8Gb downloads

Design: DNA was extracted using phenol-chloroform (Sambrook et al. 1989) and treated with RNAseA prior to library construction. Individually-barcoded TruSeq DNA libraries (Illumina) were then sequenced (100 bp paired-end).
Submitted by: University of Georgia
Study: Transcriptomes of 10 North American firefly species
show Abstracthide Abstract
Genes underlying signal reception should evolve to maximize signal detection in a particular environment. In animals, opsins, the protein component of visual pigments, are predicted to evolve according to this expectation. Fireflies are known for their bioluminescent mating signals. The eyes of nocturnal species are expected to maximize detection of conspecific signal colors emitted in the typical low-light environment. This is not expected for species that have transitioned to diurnal activity in bright daytime environments. Here we test the hypothesis that opsin gene sequence plays a role in modifying firefly eye spectral sensitivity. We use genome and transcriptome sequencing in four firefly species and transcriptome sequencing in six additional species to identify all opsin genes present in fireflies and to characterize their evolution. We also investigate expression of opsins in different tissues. Fireflies are a rich system in which to investigate the evolution of signals, receptors, and signaling environments. These resources usher these investigations into the genomic era.
Sample: Photinus pyralis whole genome shotgun sequences
SAMN03859661 • SRS993275 • All experiments • All runs
Name: 8369
Instrument: Illumina HiSeq 2000
Strategy: WGS
Selection: RANDOM
Layout: PAIRED
Spot descriptor:
forward101  reverse

Runs: 1 run, 66.5M spots, 13.4G bases, 8Gb
Run# of Spots# of BasesSizePublished


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