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SRX027140: Whole genome sequencing used to karyotype diploid, aneuploid-parented diploid and aneuploid A. thaliana individuals
1 ILLUMINA (Illumina Genome Analyzer II) run: 23.5M spots, 941.8M bases, 380.5Mb downloads

Submitted by: UC Davis
Study: Phenotypic consequences of aneuploidy in Arabidopsis thaliana
show Abstracthide Abstract
Because of their tolerance to aneuploidy, plants provide a powerful system for a genome-wide investigation of aneuploid syndromes. We phenotyped a population of Arabidopsis thaliana aneuploid individuals containing 25 different karyotypes. Even in this highly heterogeneous population, we demonstrate that certain traits are strongly associated with the dosage of specific chromosome types and that chromosomal effects can be additive. Further, we identified subtle developmental phenotypes expressed in the diploid progeny of aneuploid parent(s) but not in euploid controls from diploid lineages. These results indicate long-term phenotypic consequences of aneuploidy that can persist after chromosomal balance has been restored. We verified the diploid nature of these individuals by whole-genome sequencing and discuss the possibility that trans-generational phenotypic effects stem from epigenetic modifications passed from aneuploid parents to their diploid progeny.
Library:
Name: IH1-A.thalianaKaryotyping
Instrument: Illumina Genome Analyzer II
Strategy: WGS
Source: GENOMIC
Selection: PCR
Layout: SINGLE
Spot descriptor:
 barcode
Group tagBasecalls
Col-0#1AGCTAT
Col-0#2CATCAT
ColTr3ATGGCT
AneuploidCAGAGT
Ap2x*#10GCGACT
Ap2x*#20GTAGTT
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Runs: 1 run, 23.5M spots, 941.8M bases, 380.5Mb
Run# of Spots# of BasesSizePublished
SRR06667623,544,021941.8M380.5Mb2010-12-09

#Member# of Spots# of Bases
1SRS115091 : Col-0#14.7M188.4M
2SRS115092 : Col-0#22.9M116.9M
3SRS115093 : ColTr32M79.2M
4SRS115094 : Aneuploid2.5M100.9M
5SRS115095 : Ap2x*#104.5M178.5M
6SRS115096 : Ap2x*#206.6M263.6M
7SRS150210356,51314.3M
8 : defaultn/an/a

ID:
33001

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