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SRX020784: GSM545213: AGO2
2 ILLUMINA (Illumina Genome Analyzer) runs: 8.7M spots, 278.8M bases, 175.9Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP: sequencing data
show Abstracthide Abstract
We applied 4-thiouridine to cultured cells expressing the FLAG/HA-tagged RNA-binding proteins (RBPs) followed by UV 365 nm irradiation. The crosslinked RNA-protein complexes were isolated by immunoprecipitation, and the covalently bound RNA was partially digested with RNase T1 and radiolabeled. The radiolabeled RNPs were subsequenctly separated by SDS-PAGE, the crosslinked RNA segments recovered and converted into a cDNA library and sequenced. Overall design: RBPs were UV-crosslinked to their RNA targets containing 4-thiouridine. The RNA segments were recovered after immunoprecipitation and seqeunced by Solexa.
Sample: AGO2
SAMN00013694 • SRS073144 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GSM545213: AGO2
Instrument: Illumina Genome Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Spot descriptor:
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Experiment attributes:
GEO Accession: GSM545213
Links:
External link:
Runs: 2 runs, 8.7M spots, 278.8M bases, 175.9Mb
Run# of Spots# of BasesSizePublished
SRR0489744,588,647146.8M94.8Mb2010-05-26
SRR0489754,122,692131.9M81.1Mb2010-05-26

ID:
22300

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