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SRX020783: GSM545212: AGO1
1 ILLUMINA (Illumina Genome Analyzer) run: 4.2M spots, 133M bases, 58.8Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP: sequencing data
show Abstracthide Abstract
We applied 4-thiouridine to cultured cells expressing the FLAG/HA-tagged RNA-binding proteins (RBPs) followed by UV 365 nm irradiation. The crosslinked RNA-protein complexes were isolated by immunoprecipitation, and the covalently bound RNA was partially digested with RNase T1 and radiolabeled. The radiolabeled RNPs were subsequenctly separated by SDS-PAGE, the crosslinked RNA segments recovered and converted into a cDNA library and sequenced. Overall design: RBPs were UV-crosslinked to their RNA targets containing 4-thiouridine. The RNA segments were recovered after immunoprecipitation and seqeunced by Solexa.
Sample: AGO1
SAMN00013693 • SRS073143 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GSM545212: AGO1
Instrument: Illumina Genome Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Spot descriptor:
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Experiment attributes:
GEO Accession: GSM545212
Links:
External link:
Runs: 1 run, 4.2M spots, 133M bases, 58.8Mb
Run# of Spots# of BasesSizePublished
SRR0489734,155,911133M58.8Mb2010-05-26

ID:
22299

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