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SRX014454: GSM466495: 2'-O-methylated small RNAs from r2d2 heterozygous flies
1 ILLUMINA (Illumina Genome Analyzer) run: 13.3M spots, 478.8M bases, 364Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway
show Abstracthide Abstract
In flies, small silencing RNAs are sorted between Argonaute1 (Ago1), the central protein component of the microRNA (miRNA) pathway, and Argonaute2 (Ago2), which mediates RNA interference. Extensive double-stranded character—as is found in small interfering RNAs (siRNAs)—directs duplexes into Ago2, whereas central mismatches, like those found in miRNA/miRNA* duplexes, direct duplexes into Ago1. Central to this sorting decision is the affinity of the small RNA duplex for the Dcr-2/R2D2 heterodimer, which loads small RNAs into Ago2. Here, we show that while most Drosophila miRNAs are bound to Ago1, miRNA* strands accumulate bound to Ago2. Like siRNA loading, efficient loading of miRNA* strands in Ago2 favors duplexes with a paired central region and requires both Dcr-2 and R2D2. Those miRNA and miRNA* sequences bound to Ago2, like siRNAs diced in vivo from long double-stranded RNA, typically begin with cytidine, whereas Ago1-bound miRNA and miRNA* disproportionately begin with uridine. Consequently, some pre-miRNA generate two or more isoforms from the same side of the stem that differentially partition between Ago1 and Ago2. Our findings provide the first genome-wide test for the idea that Drosophila small RNAs are sorted between Ago1 and Ago2 according to their duplex structure and the identity of their first nucleotide. Overall design: Sequencing of small RNAs (either total small RNAs or Ago1-associated small RNAs) in wild-type, dcr-2 and r2d2 mutant flies. Small RNA sequencing, Small RNAs (18-29 nt long), Size selection (18 to 30 nt).
Sample: 2´-O-methylated small RNAs from r2d2 heterozygous flies
SAMN00006268 • SRS008442 • All experiments • All runs
Library:
Name: 2'-O-methylated small RNAs from r2d2 heterozygous flies
Instrument: Illumina Genome Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Spot descriptor:
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Experiment attributes:
GEO Accession: GSM466495
library_prep: smRNA-Seq; IP
Links:
External link:
Runs: 1 run, 13.3M spots, 478.8M bases, 364Mb
Run# of Spots# of BasesSizePublished
SRR03170213,299,937478.8M364Mb2009-12-15

ID:
14785

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