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SRX5651015: RNA-Seq of synthetically induced agarwood from trunk tissue of Aquilaria malaccensis
1 ILLUMINA (Illumina HiSeq 4000) run: 43.9M spots, 13.2G bases, 5.1Gb downloads

Design: RNA was extracted using Trizol kit according to manufacturers instruction with slight modification. Cdna libraries were prepared using NEXTNeb Ultra II RNA Library Prep Kit for Illumina. After first-strand synthesis, a custom second-strand synthesis buffer (Illumina) is added, with dNTPs, RNase H and Escherichia coli polymerase I to generate the second strand by nick-translation and AMPure XP beads is used to purify the cDNA. The final cDNA library is ready after a round of purification, terminal repair, A tailing, ligation of sequencing adapters, size selection and PCR enrichment. Library concentration was first quantified using a Qubit 2.0 fluorometer (Life Technologies), and then diluted to 1 ng/l before checking insert size on an Agilent 2100 and quantifying to greater accuracy by quantitative PCR (Q-PCR) (library activity >2 nM).
Submitted by: Universiti Kebangsaan Malaysia
Study: Transcriptome raw sequence reads of Aquilaria malaccensis
show Abstracthide Abstract
A transcriptome data of three samples plant which include healthy and two induced agarwood producing plant. We perform RNA sequencing and generatedtranscriptomic dataset of this plant elucidate the biosynthesis of terpenes in its agarwood.
Sample: Plant sample from Aquilaria malaccensis
SAMN10274164 • SRS4596195 • All experiments • All runs
Library:
Name: S
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 43.9M spots, 13.2G bases, 5.1Gb
Run# of Spots# of BasesSizePublished
SRR886360443,948,80413.2G5.1Gb2019-04-08

ID:
7614258

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