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SRX5550314: GSM3681748: Col0_3891_A: Col-0, rep1; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 21.4M spots, 6.5G bases, 2.6Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptome changes across Col-0, klu and 35S:KLU
show Abstracthide Abstract
The Arabidopsis cytochrome P450 gene KLU (also known as CYP78A5) is predicted to produce a mobile molecule that has tremendous effects on the plant growth. To gain insight the mechanism of KLU affects plant growth, we use the RNA-seq to profile the transcriptome changes across wild-type,klu mutant, and overexpression of KLU. Overall design: We use the RNA-seq to profile the transcriptom changes in the leaves across wild-type, klu mutant and overexpression of KLU.
Sample: Col0_3891_A: Col-0, rep1
SAMN11190846 • SRS4515861 • All experiments • All runs
Instrument: Illumina HiSeq 3000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: RNAs were isolated by the Total RNA was extracted using by Zymo-Spin™ IIC RNA column (Zymo Research Europe GmbH) according to manufacturer. 500 ng was used as input for polyA enrichment followed by fragmentation and library preparation as recommended by the vendor (NEBNext® Ultra™ II Directional RNA Library Prep Kit Illumina).
Experiment attributes:
GEO Accession: GSM3681748
Runs: 1 run, 21.4M spots, 6.5G bases, 2.6Gb
Run# of Spots# of BasesSizePublished


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