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SRX4899487: GSM3434641: wild type_rep3; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 22.4M spots, 6.7G bases, 1.9Gb downloads

Submitted by: NCBI (GEO)
Study: RNA-seq Analysis of the stem of Wild Type and HDT1 Transcriptomes II
show Abstracthide Abstract
RNA-seq was performed to examine the differential expressed transcriptomes in hdt1 plants during stem development compare to wild type plants. Overall design: To examine the differential expressed transcriptomes in hdt1 plants during stem development, the stem from hdt1 mutants and wild type plants at 35-days after flowering were collected for RNA-seq analysis. No replicates include in each samples.
Sample: wild type_rep3
SAMN10252433 • SRS3946012 • All experiments • All runs
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was isolated from the main stem of different developmental stage of Arabidopsis plant, using EASYspin Plant RNA kit RN09 (Aidlab, China) according to the manufacturer's instructions. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 μl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.
Experiment attributes:
GEO Accession: GSM3434641
Runs: 1 run, 22.4M spots, 6.7G bases, 1.9Gb
Run# of Spots# of BasesSizePublished


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