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SRX255057: GSM1105745: HeLa nt_control; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 85.9M spots, 12.9G bases, 8Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Muscleblind-like proteins regulate embryonic stem cell-specific alternative splicing and reprogramming II
show Abstracthide Abstract
Previous investigations of the core gene regulatory circuitry that controls embryonic stem cell (ESC) pluripotency have largely focused on the roles of transcription, chromatin and non- coding RNA regulators. Alternative splicing (AS) represents a widely acting mode of gene regulation, yet its role in the regulation of ESC pluripotency and differentiation is poorly understood. Here, we identify the Muscleblind-like RNA binding proteins, MBNL1 and MBNL2, as conserved and direct negative regulators of a large program of AS events that are differentially regulated between ESCs and other cell types. Knockdown of MBNL proteins in differentiated cells causes switching to an ESC-like AS pattern for at least half of these AS events. Among the events is an ESC-specific AS switch in the forkhead family transcription factor FOXP1 that controls pluripotency. Consistent with a central and negative regulatory role for MBNL proteins in pluripotency, their knockdown significantly enhances the expression of key pluripotency genes and the formation of induced pluripotent stem cells (iPSCs) during somatic cell reprogramming. Overall design: mRNA profiles of various embryonic stem cells, tissues and cell lines from human and mouse using high-throughput sequencing data and the role of MBNL proteins in regulation of ESC-differential alternative splicing
Sample: HeLa nt_control
SAMN01992328 • SRS404026 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Experiment attributes:
GEO Accession: GSM1105745
Links:
External link:
Runs: 1 run, 85.9M spots, 12.9G bases, 8Gb
Run# of Spots# of BasesSizePublished
SRR79705685,935,53812.9G8Gb2013-06-27

ID:
356847

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