Dor reservoir time-series sampling: 2014-03-30 - SRA

SRX4620106: Dor reservoir time-series sampling: 2014-03-30
1 ILLUMINA (NextSeq 500) run: 42.3M spots, 12.7G bases, 5.3Gb downloads

Design: Due to the decline in natural fish populations and the global increase in demand for animal-based protein, intensive aquaculture is becoming more common. The structure of the microbial ecosystem in such intensive aquaculture is of prime importance in maintaining healthy fish populations. From a theoretical point, such aquaculture facilities provide a model system to study the relative impact of anthropogenic inputs into aquatic systems, compared with the natural effects of seasonality etc. As part of a larger project studying the microbiomes from freshwater environments as they change across time and space, we sequenced metagenomes from samples collected at monthly intervals over two years at a water reservoir which is part of an aquaculture research unit at Dor, on the Mediterranean coast of Israel (3236N and 3455E). The water reservoir is part of a recirculating system which includes several production-scale fish ponds (2 hectare) and up to 81 smaller experimental ponds (0.2 hectare each). 5 liters of surface water were collected from the south shore of the reservoir using plastic jerry-cans previously sterilized by washing with 70% ethanol. The water was brought to the lab (~30 minute drive) and filtered onto GF/F filters until the filters were blocked (typically ~100 ml). DNA was extracted using a Qiacube robot, sequencing libraries were prepared using Nextera technology and sequenced on an Illumina NextSeq 500.

Submitted by: Technion - Israel Institute of Technology

Study: Time-series metagenomic sampling of the Dor water reservoir

PRJNA488159 • SRP159046 • All experiments • All runs

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Time-series shotgun metagenomic monthly sampling of the Dor water reservoir, located in the north of Israel. Sampling was carried out over the course of two years, between 2013-2014. A total of 23 samples are included in the dataset.

Sample:

SAMN09925779 • SRS3722663 • All experiments • All runs

Organism: metagenome

Library:

Name: DS-RMar4

Instrument: NextSeq 500

Strategy: WGS

Source: METAGENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 42.3M spots, 12.7G bases, 5.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR7764480	42,296,733	12.7G	5.3Gb	2019-09-23

ID:: 6242021

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