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SRX3908988: GSM3090185: col0_fb_3.mRNAseq; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 24.6M spots, 1.2G bases, 440.6Mb downloads

Submitted by: NCBI (GEO)
Study: nanoPARE: Parallel analysis of RNA 5' ends from low input RNA
show Abstracthide Abstract
A diversity of RNA molecule 5' ends are generated during transcriptional and post-transcriptional processes. Different RNA ends can confer or represent different functional activities and thus the identification of RNA end usage dynamics contributes to the functional characterization of RNA molecules. Here we present a method that enables the accurate identification of RNA 5' ends from samples with low amounts of total RNAs, and thus allow characterization of RNA regulatory mechanisms in specific cell-types. Overall design: NanoPARE, or RNA 5' end enrichment, libraries were constructed for three biological replicates of ten conditions/genotypes. mRNA-Seq libraries were generated were constructed for three biological replicates of two conditions/genotypes.
Sample: col0_fb_3.mRNAseq
SAMN08897825 • SRS3144734 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: TRIzol reagent (Thermo Fisher Scientific) For Samples 1-6, mRNA-Seq libraries were generated according to the Smart-Seq2 protocol (Picelli et al. 2013. Nature Methods). For Samples 7-30, nanoPARE libraries were generated as detailed in the associated article.
Experiment attributes:
GEO Accession: GSM3090185
Links:
Runs: 1 run, 24.6M spots, 1.2G bases, 440.6Mb
Run# of Spots# of BasesSizePublished
SRR696708024,626,0641.2G440.6Mb2018-12-04

ID:
5365804

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