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SRX3532623: GSM2913049: WT mRNA-seq rep3; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 25.2M spots, 2.5G bases, 788.2Mb downloads

Submitted by: NCBI (GEO)
Study: mRNA sequencing of 4 week old leaves in Arabidopsis wild type and ABI3:MTA
show Abstracthide Abstract
In order to gain insight into abundance of transcripts in the presence and absence of m6A, we performed an mRNA sequencing expirement in both mta and Col-0 plants. Overall design: 2 replicates per genotype of miCLIP-seq in Arabidopsis thaliana ecotype Col-0 wild-type (WT) and ABI3:MTA (mta).
Sample: WT mRNA-seq rep3
SAMN08292363 • SRS2808061 • All experiments • All runs
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: Leaves 5-9 were collected 4 hrs after first light. Leaves were immediately placed on liquid N2 and ground using a mortar and pestle. RNA was extracted using miRNeasy mini kit (Qiagen) according to manufacturers instructions. Strand specific libraries were constructed using the TruSeq mRNA sample library preparation (Truseq) as described in the manual.
Experiment attributes:
GEO Accession: GSM2913049
Runs: 1 run, 25.2M spots, 2.5G bases, 788.2Mb
Run# of Spots# of BasesSizePublished


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