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SRX3415618: GSM2863380: WT-B_RNAseq; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 40.6M spots, 12.2G bases, 4Gb downloads

Submitted by: NCBI (GEO)
Study: INDUCER OF CBF EXPRESSION 1 is a male fertility regulator impacting anther dehydration in Arabidopsis
show Abstracthide Abstract
Our results show that ICE1 controls plant male fertility via impacting anther dehydration. The loss-of-function mutation in ICE1 gene in Arabidopsis caused anther indehiscence and decreased pollen viability as well as germination rate. Further analysis revealed that the anthers in the mutant of ICE1 (ice1-2) had the structure of stomium, though the epidermis did not shrink to dehisce. The anther indehiscence and influenced pollen viability as well as germination in ice1-2 were due to abnormal anther dehydration, for most of anthers dehisced with drought treatment and pollen grains from those dehydrated anthers had similar viability and germination rates compared with wild type. Accordingly, the sterility of ice1-2 could be rescued by ambient dehydration treatments. Likewise, the stomatal differentiation of ice1-2 anther epidermis was disrupted in a different manner compared with that in leaves. ICE1 specifically bound to MYC-recognition elements in the promoter of FAMA, a key regulator of guard cell differentiation, to activate FAMA expression. Transcriptome profiling in the anther tissues further exhibited ICE1-modulated genes associated with water transport and ion exchange in the anther. Together, this work reveals the key role of ICE1 in male fertility control and establishes a regulatory network mediated by ICE1 for stomata development and water movement in the anther. Overall design: mRNA profiles of wild type (Col-0) and ice1-2 -/- Arabidopsis were generated by deep sequencing, in triplicate.
Sample: WT-B_RNAseq
SAMN08057457 • SRS2711687 • All experiments • All runs
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from collected stage 9-13 anther using ZR Plant RNA MiniPrepTM Kit (ZYMO Research Corp., USA) and purified using RNAeasy Plant Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's protocol. The constructed libraries were sequenced using the HiSeqTM 2000 Sequencing System (Illumina, San Diego, CA, USA) with single-end technology in a single run at Shanghai Genergy Biotechnology (Shanghai) Co., Ltd. (Shanghai, China) and subjected to 100 cycles of paired-end (2×100 bp) sequencing.
Experiment attributes:
GEO Accession: GSM2863380
Runs: 1 run, 40.6M spots, 12.2G bases, 4Gb
Run# of Spots# of BasesSizePublished


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