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SRX196288: GSM1020664: mouse_c_spleen; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 113.3M spots, 11.3G bases, 6.8Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Evolutionary dynamics of gene and isoform regulation in mammalian tissues
show Abstracthide Abstract
Most mammalian genes produce multiple distinct mRNAs through alternative splicing, but the extent of splicing conservation is not clear.  To assess tissue-specific transcriptome variation across mammals, we sequenced cDNA from 9 tissues from 4 mammals and one bird in biological triplicate, at unprecedented depth.  We find that while tissue-specific gene expression programs are largely conserved, alternative splicing is well conserved in only a subset of tissues and is frequently lineage-specific. Thousands of novel, lineage-specific and conserved alternative exons were identified; widely conserved alternative exons had signatures of binding by MBNL, PTB, RBFOX, STAR and TIA family splicing factors, implicating them as ancestral mammalian splicing regulators.  Our data also indicates that alternative splicing is often used to alter protein phosphorylatability, delimiting the scope of kinase signaling. Overall design: Tissue transcriptomes from 9 tissues from 5 species, 3 individuals per species, were sequenced and compared (two samples for mouse_heart)
Sample: mouse_c_spleen
SAMN01766838 • SRS369725 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Experiment attributes:
GEO Accession: GSM1020664
External link:
Runs: 1 run, 113.3M spots, 11.3G bases, 6.8Gb
Run# of Spots# of BasesSizePublished


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