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SRX2536516: GSM2476074: seed following 48 h of stratification (S) Replicate 2 [RNA-seq]; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 1000) run: 75.6M spots, 4.5G bases, 3Gb downloads

Submitted by: NCBI (GEO)
Study: RNA-seq data over Arabidopsis thaliana germination
show Abstracthide Abstract
RNAseq profiling of 10 time points during germination in Arabidopsis, from freshly harvested seed, through mature seed, stratification, germination and to post-germination. Overall design: Total RNA was extracted from Arabidopsis seeds at 10 time points during germination in triplicate. The time points were: freshly harvested seed (H), seeds following 15 days of ripening (0 h), seeds after; 1 h of stratification (1 h S), 12 h of stratification (12 h S), 48 h of stratification (48 h S), followed by seed collected 1 hour into the light (1 h SL), 6 hours into the light (6 h SL), 12 hours into the light (12 h SL), 24 hours into the light (24 h SL) and 48 hours into the light (48 h SL).
Sample: seed following 48 h of stratification (S) Replicate 2 [RNA-seq]
SAMN06293590 • SRS1956456 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 1000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA isolation was carried out using the Ambion Plant RNA isolation aid and RNAqeous RNA isolation kit. RNA-seq libraries were generated using the TruSeq Total RNA kit v2 according to manufacturer’s instructions (Illumina). polyA selection, random fragmentation, unstranded library
Experiment attributes:
GEO Accession: GSM2476074
Links:
Runs: 1 run, 75.6M spots, 4.5G bases, 3Gb
Run# of Spots# of BasesSizePublished
SRR522740175,569,5844.5G3Gb2017-08-16

ID:
3669909

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