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SRX2531707: GSM2474227: siCTL_N1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 69.2M spots, 17.3G bases, 8.4Gb downloads

Submitted by: NCBI (GEO)
Study: downregulation of DDX5 and DDX17
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RNAseq : MCF-7 cells were transfected with siRNA targeting both DDX5 and DDX17 RNA helicases, and total RNA were extracted as described previously (Dardenne Cell Rep 2014). Overall design: siRNA control (2 replicates) + siRNA DDX5/DDX17 (2 replicates)
Sample: siCTL_N1
SAMN06288072 • SRS1952538 • All experiments • All runs
Organism: Homo sapiens
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: total RNA were extracted as described previously (Dardenne Cell Rep 2014) RNA libraries were prepared for sequencing using standard Illumina protocols. rRNA were depleted with the "TruSeq Stranded Total RNA with Ribo‐Zero Gold" kit.
Experiment attributes:
GEO Accession: GSM2474227
Runs: 1 run, 69.2M spots, 17.3G bases, 8.4Gb
Run# of Spots# of BasesSizePublished


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