GSM2438756: Day 16 replicate 2; Rattus norvegicus; RNA-Seq - SRA

SRX2441420: GSM2438756: Day 16 replicate 2; Rattus norvegicus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 10.6M spots, 531.3M bases, 324.6Mb downloads

Submitted by: NCBI (GEO)

Study: Gene expression profiling of cultured embryonic rat cortical neurons

PRJNA358621 • SRP095569 • All experiments • All runs

show Abstracthide Abstract

Embryonic CNS neurons can differentiate in culture and provide a model for maturation-related changes. A transcriptome profile over the course of maturation was perform to investigate the underlying molecular mechanism that drives maturation and its related changes such as developmental loss of intrinsic regenerative capacity. Overall design: Primary cortical neurons were excised from E18 rat embryos and were co-cultured with primary rat astrocytes from P0-2 rats. RNA were then extracted on day 1, 4, 8, 16, and 24 after plating.

Sample: Day 16 replicate 2

SAMN06176811 • SRS1876087 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted from 1, 4, 8, 16, and 24 DIV cultures by using the Qiagen RNeasy Micro Kit (Qiagen, 74004) RNA was subjected to whole-transcriptome amplification using NuGEN RNAseq v2 System followed by fragmentation to 200bp with a Diagenode Bioruptor Pico sonicator, The resulting fragments were then used to make bar-coded libraries using NuGEN Ovation Rapid DR Multiplex System

Experiment attributes:

GEO Accession: GSM2438756

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 10.6M spots, 531.3M bases, 324.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR5127143	10,626,662	531.3M	324.6Mb	2017-06-05

ID:: 3544883

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