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SRX2416971: GSM2425537: Drosophila melanogaster w1118 testis total RNA replicate 2; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 28M spots, 2.8G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)
Study: Characterization of a TUTase/nuclease complex required for Drosophila gametogenesis
show Abstracthide Abstract
Total RNAs were cloned from wt, Dis3L2 and Tailor mutant testis tissues to study the role of Tailor and Dis3L2 TUTase/nuclease complex Overall design: Replicated total RNA samples from Dis3L2 and Tailor single mutant, and Dis3L2/Tailor double mutant and WT (w1118) testes
Sample: Drosophila melanogaster w1118 testis total RNA replicate 2
SAMN06134386 • SRS1854357 • All experiments • All runs
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNAs were isolated from w1118, Tailor, Dis3L2, and Tailor/Dis3L2 mutant 5 day old fly testes using Trizol. Two independent dissections were made to generate biologically replicate RNA samples, whose quality was assessed by Bioanalyzer The Illumina Truseq Total RNA library Prep Kit LT was used to make RNAseq libraries from 650 ng of total RNA. Manufacturer’s protocol was followed except for using 8 cycles of PCR to amplify the final library instead of the recommended 15 cycles, to minimize artifacts caused by PCR amplification. All samples were pooled together, using the barcoded adapters provided by the manufacturer, over 2 flow cells of a HiSeq2500 and sequenced using pair-end PE50 at the New York Genome Center.
Experiment attributes:
GEO Accession: GSM2425537
Runs: 1 run, 28M spots, 2.8G bases, 1.3Gb
Run# of Spots# of BasesSizePublished


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